Hardik K. Soni*, Bhavik R. Shah2, Surendra B. Bhatt1, Navin R. Sheth²

1. Vasu Research Centre, A Division of Vasu Health care Pvt. Ltd., 896/A, G.I.D.C., Makarpura,Vadodara-390010, Gujarat, India

2.  Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360005, Gujarat, India

Received on: 19-04-2010; Revised on: 12-05-2010; Accepted on:17-07-2010

Abstracts:

Herbal supplements are more popular for improvement of primary healthcare as they are turning to natural remedies. Looking to this aspect, determination of nutritional value is essential for every herbal supplements. In this research work an attempt was made to establish system of standardization of herbal supplements like manjistha, kokam, and punarnava in order to assess the quality of the drugs. Powder characteristic and various quality control parameters were performed. Data of quality control parameters showed that the extracts used in formulation were of good in quality. HPTLC analysis indicates all extracts were derived from genuine parts of the plant. Concentration of lead, arsenic, cadmium and mercury in selected capsules and extract passes the limit for heavy metal content. Microbial analysis data revealed that Manjistha, Kokam and Punarnava had shown < 10 cfu/gm total bacterial counts. Total yeast and mould count, and the pathogens like E. coli, Salmonella, P. aeruginosa and S. aureus were absent in all selected capsules. Data of Nutritional value revealed that Manjistha capsule contain carbohydrate 93.82%, protein 3.06% and cholesterol 2.58%. While Kokam, and Punarnava capsule contain carbohydrate 86.60% and 83.13%, protein 3.24% and 7.19% and cholesterol 2.62% and 2.51%, respectively. From the obtained data we concluded that all the selected capsules and extracts were consistent with various quality and purity parameters. Nutritional assessments of the each capsule indicate all the drugs have good dietary supplement value and medicinal role as an herbal supplement.

Key words: Herbal supplements, Nutritional assessment, HPTLC, Heavy metal

Hardik K. Soni 1*, Pooja A. Kanasagara2, Surendra B. Bhatt1, Urvashi D. Shah 2, Vaishali N. Shah ²

Abstracts:

Standardization of polyherbal formulation – Acnovin Active Syrup (Blood Purifier) Standardization of Polyherbal formulation is essential in order to assess the quality of drugs, based on the concentration of their active principles. This research paper reports on Standardization of Acnovin Active Syrup (Blood purifier) which is Polyherbal formulation used as purifies the blood and aids in overcoming problems, Acne and Boils. It also helps in other skin related disorders, which was manufactured by Vasu Healthcare Pvt. Ltd. Standardization protocol included different parameters for authentification of raw Material as well as finished product such as Identification of Raw Materials, Organoleptic evaluation, Physicochemical parameters such as pH value, Viscosity, Weight/ml, Total sugar content, Phenolic content, Total Ash, Acid insoluble Ash, Water soluble Ash, Water and Alcohol soluble Extractive value, Qualitative Phytochemical evaluation, HPTLC Fingerprintin of finished product, Quantification of Curcumin and Gallic Acid in Syrup, Heavy metal analysis, Microbial analysis was done. The data generated from the present study would help in the authentification of the drug and the details analysis with the help of Determination of sugar content, Determination of phenolic content, HPTLC Fingerprinting, Heavy metal analysis, Microbial analysis will be useful in the standardization of the Polyherbal formulation.

Key Words: Acnovin Active Syrup, Phenolic content, HPTLC Fingerprinting, Heavy metal analysis, Microbial analysis.

Soni Hardik K1*, Ribadiya Nikunj C2, Bhatt Surendra B1, Sheth Navin R²

1.Vasu Research Centre, A Division of Vasu Health care Pvt. Ltd., 896/A, G.I.D.C., Makarpura Vadodara-390010, Gujarat, India

2.  Department of Pharmaceutical Sciences, Saurashtra University, Rajkot-360005, Gujarat, India

Abstract:

The most important challenges faced by herbal formulations arise because of their lack of complete evaluation. Evaluation is necessary to ensure quality and purity of the herbal product. For evaluation of capsule containing single herb various parameters were tested. These parameters for raw material include powder characteristic study, organoleptic, physicochemical, phytochemical parameters etc., and assay of active constituent. Parameters for finished product (capsule) include uniformity of weight, pH, moisture content, disintegration time and dissolution study. HPTLC study, heavy metal analysis, microbial analysis and Nutritional value were carried out as a part of evaluation. Results indicate that Ashwagandha capsule has passed through all organoleptic and physicochemical parameters. Active constituent was present in adequate amount in the Ashwagandha capsule. Data of HPTLC finger printing indicates that extract was derived from genuine plant or parts of the plant and also capsule contain the same extract. Concentration of lead, arsenic and cadmium in capsules passed the limit of heavy metal. Mercury was absent in capsule and in their extract. Capsules have <10 cfu/gm total bacterial count. Total yeast and mould was absent in capsules. The pathogens like E. coli, Salmonella, P.aeruginosa and S.aureus were also absent in capsule.Carbohydrate, protein and cholesterol content for Ashwagadha capsule is 84.56%, 8.29% and 2.32% respectively. Data suggested that capsule and its extract were consistent with various identity, quality and purity parameters such as organoleptic characters, physico-chemical parameter, HPTLC fingerprinting, heavy metal and microbial analysis. Nutritional assessment of the each capsule indicates their dietary supplement value.

Keywords: Withania somnifera, Herbal Formulation, HPTLC fingerprinting, Nutritional value, Heavy metal & Microbial analysis.

Aghera B., Patel N. M., Bhatt S.B.*, Parekh J., Makwana H. and Solanki B.
Received 04 September 2009, Accepted 16 February 2010.

Abstracts:

Standardization of two anti diabetic herbal formulations Glucova Tablet and Glucova Capsule were done which is manufactured by Vasuhealthcare Pvt.Ltd. Standardization protocol included different parameters for authentication of raw material such as identification of raw materials, organoleptic parameters like colour/ odour/ taste; physicochemical parameters such as pH value, bitterness value, qualitative phytochemical evaluation, HPTLC finger-printing and heavy metal analysis was done. For standardization of finished products parameters studied included uniformity of weight, measurement of diameter and thickness, measurement of hardness, determination of pH, disintegration test, dissolution test, moisture content, HPTLC finger-printing, heavy metal analysis and and microbial analysis. The data generated from the present study would help in the authentication of the drug and the details analysis with the help of HPTLC fingerprint profile will be useful in the standardization of the herbal formulation.

Keywords: Glucova Tablet/Capsule, Standardization, HPTLC fingerprint, Heavy Metal analysis, Microbial analysis.

Acharya Mayank J.², Soni Hardik K.¹, Bhavik R. Shah1, Patel Rakesh K.², Bhatt Surendra B.¹

1. Vasu Research centre, A Division of Vasu Healthcare Pvt. Ltd., 896/A G.I.D.C. Makarpura,Vadodara- 390010, Gujarat, India

2. S.K.Patel College of pharmaceutical Education and Research, Ganpat University, Kherva-382711, Mehsana, Gujarat, India

E-mail Address: thegreekgod004m@yahoo.com

Abstracts:

Herbal supplements are more popular for improvement of primary healthcare as they are turning to natural remedies. Looking to this aspect, determination of nutritional value is essential for every herbal supplements1. In this research work an attempt was made to establish system of standardization of herbal supplements like manjistha, kokam, and punarnava in order to assess the quality of the drugs. Powder characteristic and various quality control parameters were performed. Data of quality control parameters showed that the extracts used in formulation were of good in quality. HPTLC analysis indicates all extracts were derived from genuine parts of the plant. Concentration of lead, arsenic, cadmium and mercury in selected capsules and extract passes the limit for heavy metal content. Microbial analysis data revealed that Manjistha, Kokam and Punarnava had shown < 10 cfu/gm total bacterial counts. Total yeast and mould count, and the pathogens like E. coli, Salmonella, P. aeruginosa and S. aureus were absent in all selected capsules. Data of Nutritional value revealed that Manjistha capsule contain carbohydrate 93.82%, protein 3.06% and cholesterol 2.58%. While Kokam, and Punarnava capsule contain carbohydrate 86.60% and 83.13%, protein 3.24% and 7.19% and cholesterol 2.62% and 2.51%, respectively. From the obtained data we concluded that all the selected capsules and extracts were consistent with various quality and purity parameters. Nutritional assessments of the each capsule indicate all the drugs have good dietary supplement value and medicinal role as an herbal supplement.

Thakker Tejaskumar J.², Aghera Bina¹, Soni Hardik K.¹, Patel Rakesh K.²

1. Vasu Research centre, A Division of Vasu Healthcare Pvt. Ltd., 896/A G.I.D.C. Makarpura,Vadodara- 390010, Gujarat, India

2. S.K.Patel College of pharmaceutical Education and Research, Ganpat University, Kherva-382711, Mehsana, Gujarat, India

E-mail Address: thakkertj_2006@yahoo.co.in

Abstracts:

There are many diseases that are caused due to genetic disorders and Diabetes is one of them. Diabetes is a disorder of metabolism-the way our body use digestive food for growth and energy. There are more than 125 million people with diabetes in the world today1. So the proprietary herbal formulation of Vasu Pharmaceutical Pvt.Ltd. i.e. Glucova Tablet/capsule was evaluated for anti diabetic activity. Antidiabetic activity is assessed by alloxan induced diabetic rats with comparison to the standard reference drug marketed with the brand name Glebenclemide. Glucova tablet and Glucova capsule had shown significant antidiabetic activity in alloxan induced diabetic rats with comparison to the standard reference drug marketed with the brand name Glebenclemide for various parameters such as Body weight, Blood glucose, Serum cholesterol, Serum triglyceride, Serum HDL, Serum VLDL, Serum LDL, Serum urea, Serum creatinine, Serum ALPH, Serum ALT, Serum AST, Serum bilirubin, Serum total protein and albumin. It was observed to have almost parallel values for all the biochemical parameters estimated in comparison to Reference Standard drug- Glebenclemide. Furthermore, the histopathological analysis of Pancreas showed that the herbal formulations Glucova capsule and Glucova tablet retained the tissue degeneration in comparison to the Diabetic Control group.

Bhavna Solanki, Kruti Pandya, Kunal Maniar and Nilesh Gurav, Surendra B. Bhatt
¹Vasu Research Centre, A Division of Vasu Healthcare Pvt. Ltd., Vadodara, India

The different plant extracts were initially screened for their anti microbial activity and then, were extracted in different solvents. The extracts were tested against two Gram positive and four Gram negative organisms by using the standard disc diffusion method. Phytochemical analysis were also carried out that included Assay of Withanoloides for Withania somnifera (Ashwagandha) and Assay of Tannin and Saponin for Hemidesmu indicus (Sariva) and Assay of Gingerol in Zingiber officinale (Shunthi Rhizome). The actives of Zingiber officinale were also confirmed by the HPLC method. They were also screened for the presence of both Heavy metals and microbial analysis by standard protocols.

Bhavna Solanki, Kruti Pandya, Kunal Maniar and Nilesh Gurav, Surendra B. Bhatt

Nutritional deficiency is almost impossible to avoid in these modern times, thus supplements help us to overcome the nutritional deficiencies. It also helps us to boost our immune system. Nutritional supplements are also useful in getting rid of the toxins that are accumulated in our body. Thus the five natural supplements that are mentioned below are tested for the various parameters that include the basic Quality Control Parameters, the phyto chemical analysis, Microbial Analysis that includes the testing for the presence of pathogens along with the total bacterial and fungal count. It is also tested for the presence of heavy metals in them, followed by Aflatoxin and Pesticide analysis. The Nutritional Value for each of them were determined and reported in the form of mg/capsule. The actives of Licorice Capsule Mucuna Capsule and Triphala Capsule were confirmed by the HPLC method where as that of Shatavari was confirmed by the HPTLC Method.

Abstracts:

The novel poly herbal formulation i.e, Bonton Active Granules was prepared such that it acts on the deficiency of bones thus helping in Osteoporosis. It contains different extracts that helps in improving bone strength and healing bones. The efficacy and the nutritional value of the poly herbal formulation was determined and reported that signifies the formulation. Estrogen deficiency is known to play a critical role in the development of osteoporosis, while calcium and vitamin D deficiencies and secondary hyperparathyroidism also contribute in it. The formulation was also checked for the presence of microbial pathogens, and for the presence of heavy metals in it. The study also included various risk factors symptoms and the tests that are done to detect Osteoporosis.

Standardization of Emblica Officinalis capsule and its raw material have been carried out in the present study. The study includes antimicrobial evaluation along with estimation of its physicochemical parameters such as ash, extractive values and preliminary phytochemical screening. It also includes quantification of some of the active constituents like Tannins, Gallic acid, and Vitamin C. Study shows Vitamin C, Gallic Acid and Tannin in capsule were 6.32%, 9.96% and 24.12% respectively. Emblica Officinalis shows good anti-microbial activity against E.coli, Salmonella and S.aureus. It reveals standardization profile for drug like Emblica officinalis, which would be of immense value in botanical identification and authentification of plant drug and may help us in preventing its adulteration.

Nutritional deficiency is almost impossible to avoid in these modern times, thus natural supplements help us to overcome the nutritional deficiencies. It also helps us to boost our immune system. Nutritional supplements are also useful in getting rid of the toxins that are accumulated in our body. Thus, the five natural supplements that are mentioned below are tested for the various parameters that include the basic Quality Control Parameters, the phyto chemical analysis, Microbial Analysis that includes the testing for the presence of pathogens along with the total bacterial and fungal count. It is also tested for the presence of heavy metals in them, followed by Aflatoxin and Pesticide analysis. The Nutritional Value for each of them were determined and calculated. The actives of Garcinia Capsule, Ginger Capsule and Holy Basil Capsule were confirmed by the HPLC method.

Acnovin Capsule has a good amount of herbal ingredients that possess antimicrobial activity. They were screened to show their anti bacterial activity in three solvent extractions. They were also screened for the presence of pathogens, heavy metals and their Quality Control Parameters. All the ingredients have good activity on the skin. Few of the samples were also tested for their HPLC and HTLC charcaterization.

¹S.K.Patel College Of Pharmaceutical Education & Research, Ganpat University, Kherva-382711, Mehsana, Gujarat, India

²Vasu Research Centre, A Division Of Vasu Healthcare Pvt. Ltd., 896/A G.I.D.C. Makarpura,Vadodara- 390010, Gujarat, India

E-mail Address: jitsmodi@yahoo.com

Standardization of herbal formulations is essential in order to assess the quality, purity, safety and efficacy of drugs based on the amounts of their active principles. The present research work is an attempt to standardize “herbal granules ” a marketed polyherbal formulation used in the treatment of Sinusitis, tonsillitis, nasal congestion, throat congestion, broncho-spasm, bronchitis, dyspnoea, breathlessness, mild state of bronchial asthma and other upper, lower respiratory congestion and other respiratory system related problems.. The Raw material and formulation were standardized on the basis of organoleptic characters, physical characteristics and physico-chemical properties. Also quantitative evaluation of active marker compound was done in raw material and final formulation. The set parameters were found to be sufficient to evaluate the granules and can be used as reference standards for the quality control/quality assurance purposes in future.

¹S.K.Patel College of pharmaceutical Education and Research, Ganpat University, Kherva-382711, Mehsana, Gujarat, India

²Vasu Research Centre, A Division of Vasu Healthcare Pvt. Ltd., 896/A G.I.D.C. Makarpura,Vadodara- 390010, Gujarat, India

E-mail Address: thegreekgod004m@yahoo.com

Rheumatoid arthritis is an inflammatory autoimmune disease that causes pain, swelling, stiffness and loss of function in the joints1. Most of the available NSAIDs are effective but devoid of gastro protective properties. Dazzle capsule is a polyherbal formulation containing dry extracts of herbs like ashwagandha, sunth, nagarmoth, guggulu, nirgundi, guduchi etc. In this research work we have made an attempt to establish the standardization theme for polyherbal capsule formulation. Results obtained from quality control study showed that the extracts were good in quality. Concentration of lead, arsenic, cadmium and mercury in selected capsules and extracts passes the limit for heavy metal content. Microbial analysis data showed that selected capsule samples and extracts had < 100 cfu/gm total bacterial counts. Total yeast and mould count, and the pathogens like E. coli, Salmonella, P. aeruginosa and S. aureus were absent in all selected extract and capsule samples. Assay for the respective chemical composition of all herb extracts were done and result shows the satisfactory data within limits. From these obtained data we concluded that all the selected capsules and extracts were consistent with various quality and purity parameters. Further study is going on for assessment of safety and efficacy of the formulation.

1) School of Pharmaceutical Science, Venue of Seedling Group of Institute, Jaipur National University, Jagatpura, Jaipur ,Rajasthan, India

E-mail Address: krunal.ukani@gmail.com

Alopecia is a dermatological disorder with psychosocial implications on patients with hair loss.1 Hair loss is common & ever increasing problem in cosmetics as well as primary health care problem worldwide.2 Herbal formulations always have attracted considerable attention because of their good activity and comparatively lesser or negligible side effects with synthetic drugs. The objective of present study involves preparation of herbal hair oil using Sesam oil ,coconut oil, Neem ,Bhringraj, Amala, Mandukaparini, Nilpuspa, Nagarmoth, Datura, Gunja, Jatichetika etc. and its evaluation for increase in hair growth activity. In this research work an attempt was made to establish system of standardization of herbal extracts & oils in order to assess the quality of the Product. Powder characteristic and various quality control parameters were performed. Data of quality control parameters showed that all extracts & based oils used in formulation were of good in quality. TLC analysis indicates all extracts were derived from genuine parts of the plant. Phytochemical analysis was performed for quantification of active constituents present in raw extracts used in formulation. Concentration of Lead, Arsenic, Cadmium and Mercury in raw materials and formulation passes the limit for heavy metal content. Microbial analysis data revealed that pathogens like E. coli, Salmonella, P. aeruginosa and S. aureus were absent in raw materials & finished product. From the obtained data we concluded that all the raw materials & formulation consist good quality. Further study is going on for the assessment of safety & efficacy of the formulation.

Emergence of multi-drug resistant (MDR) and extensivelydrug resistant (XDR) strains of Mycobacterium tuberculosis has further complicated the problem of tuberculosis (TB). It is thus very essential to find out a remedy for this killer disease. In this article which is a preliminary study to show that certain essential oils possess anti-mycobacterial activity. In this study, essential oil of few Ayurvedic plants like Devdaru, Shigru, Karanj and Neem have been screened individually and in combinations for their anti-mycobacterial activity and promising results were found against the Mycobacterium smegmatis, a non-pathogenic species of Mycobacterium group. Devdaru oil shows the best result compared to the other oils tested. In continuation to this preliminary work, further analysis and research is in progress.

Standardization of Glucova Active tablet, a herbo mineral formulation is essential in order to assess the quality of drugs, based on the concentration of their active principles. The formulation has a very good effect on Diabetes and the protocol included different parameters for authentification of raw material as well as finished product. HPLC and HPTLC were also carried put to authenticate the drugs along with other parameters like Microbial analysis, heavy metal analysis etc. the data got thus suggests that all the ingredients are useful in the formulation and have good reported activity against diabetes.

Standardization of a product is very essential in order to assess the quality of the drugs. All the tests carried out in this research shows that the drug/extract is of good quality. The samples were tested for their basic Physico-chemical parameters and were much within the limits specified. They were also checked for the presence of contaminants viz; microbial and traces of heavy metals and they were free of both the contaminants. They were also screened for their anti microbial activity in two different varying concentrations and both of them showed to possess good anti-microbial activity against the pathogens tested. The assessment of the same showed that the two capsules showed good anti microbial activity at varying concentrations and were of standard quality.